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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Nucleosides Nucleotides Nucleic Acids. 2011 Apr;30(4):271–279. doi: 10.1080/15257770.2011.580640

FIGURE 2.

FIGURE 2

Comparison of these two DMTr-deprotection strategies on 5'-DMTr-AT-6-SeG-TCG-3' (SM). Profile a: SM; Profile b: product (P) after the deprotection; Profile c: co-injection. HPLC gradient for SM: runs from 5 to 60% buffer B in 8 min and then from 60 to 100% buffer B in 2 min. Gradient for P: from 5 to 40% buffer B in 20 min and then from 40 to 100% buffer B in 3 min. Gradient for co-injection: from 5 to 40% buffer B in 17 min and then from 40 to 100% buffer B in 3 min. A) using the conventional strategy (treatment with 3% CCl3COOH for 3 min before neutralization); B) using the mild detritylation strategy (heating at 40 °C and pH 4.5 for 1 hr).