Klenow fill in for pYD(+1)	Mung bean chew back for pYD(−1)
3 μL 10x React 2	3 μL 10x Mung bean reaction buffer
2 μL 0.5 mM dNTP mix	20 μL Linearized pYD1 (≈2 μg)
20 μL Linearized pYD1 (≈2 μg)	1 μL Mung bean nuclease (10U/μL)
2 μL Klenow fragment (5U/ul)	6 μL ddH20
4 μL ddH20	Incubate at 30°C for 30 min
Incubate at RT for 30 min