| 3 μL 10x React 3 | 5 μL 10x React 3 |
| 10 μg pYD1 plasmid | 20 μg cDNA library plasmid |
| 2 μL EcoRI | 2 μL EcoRI |
| Bring volume to 30 μL with ddH2O | Bring volume to 50 μL with ddH2O |
| Incubate at 37°C for 3 h. |
| 3 μL 10x React 3 | 5 μL 10x React 3 |
| 10 μg pYD1 plasmid | 20 μg cDNA library plasmid |
| 2 μL EcoRI | 2 μL EcoRI |
| Bring volume to 30 μL with ddH2O | Bring volume to 50 μL with ddH2O |
| Incubate at 37°C for 3 h. |