Table 3.
# of probes in hyb. mix | Genes represented by probes in hyb. mix | Probe typea | # of pos. clonesb | Library addresses of positive clonesc | Mean hits per genome |
---|---|---|---|---|---|
5 | Chi1, Ferr, Pat, HemA, Lcyb | OVG | 7 | 1052C3d, 1069I8d, 1137O7d, 1227H24, 1258G13, 1271L2, 1276E22 | 1.4 |
8 | AMT, AQU, Cal, Chi1, Cryj2, Ferr, Lcyb, HemA | PCR | 12 | 721G13, 724I23, 738H3, 749O14, 775F3, 813M13, 822N2, 826H4, 835L14, 836I14, 864F18, 885D20 | 1.5 |
aOVG = overgo (22-mer); PCR = PCR amplicon (each ca. 500 bp)
bPer one genome equivalent, i.e., per 73,728 clones
cClone addresses are composed of a plate number, a row letter, and a column number, e.g., 1052N22 is the clone found in plate 1052, row N, column 22.
dA dot-blot experiment verified that clones 1052N22, 1069H17, and 1137O7 contained the HemA, Chi1, and Pat genes, respectively. Specific clone/probe relationships were not determined for the other clones.