Table 3.

Interrogation of macroarrays with overgo and PCR amplicon gene-specific probes

# of probes in hyb. mix	Genes represented by probes in hyb. mix	Probe typea	# of pos. clonesb	Library addresses of positive clonesc	Mean hits per genome
5	Chi1, Ferr, Pat, HemA, Lcyb	OVG	7	1052C3d, 1069I8d, 1137O7d, 1227H24, 1258G13, 1271L2, 1276E22	1.4
8	AMT, AQU, Cal, Chi1, Cryj2, Ferr, Lcyb, HemA	PCR	12	721G13, 724I23, 738H3, 749O14, 775F3, 813M13, 822N2, 826H4, 835L14, 836I14, 864F18, 885D20	1.5

^aOVG = overgo (22-mer); PCR = PCR amplicon (each ca. 500 bp)

^bPer one genome equivalent, i.e., per 73,728 clones

^cClone addresses are composed of a plate number, a row letter, and a column number, e.g., 1052N22 is the clone found in plate 1052, row N, column 22.

^dA dot-blot experiment verified that clones 1052N22, 1069H17, and 1137O7 contained the HemA, Chi1, and Pat genes, respectively. Specific clone/probe relationships were not determined for the other clones.