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Effect of cofactors on Vmax of ME activity
| Addition |
Km for malate (mM) |
Vmax nmol × (min × mg protein)-1 |
p-value to controls |
|---|---|---|---|
| None | - | 3 ± 1 | - |
| MgCl2 (3 mM) | - | 24 ± 2 | 0.02 |
| MnCl2 (3 mM) | 0.12 | 56 ± 3 (20 2) | 0.01 |
All kinetic parameters were obtained with NAD+ except the Vmax obtained with NADP+ (value in parentheses). The Vmax values were expressed as mean ± SEM. All other experimental details are described in the Methods.
