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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Cell Physiol. 2012 May;227(5):2145–2153. doi: 10.1002/jcp.22947

Figure 2. Ad.5/3-mda-7 induces MDA-7/IL-24 expression and enhances killing in low CAR expressing RKO cancer cells.

Figure 2

A and B. HCT116 and RKO cells were infected with the indicated doses of Ad.5-mda-7 or Ad.5/3-mda-7 and respective controls. After 48 hr of infection total proteins were isolated. The expression of MDA-7/IL-24, activation of PARP (as a marker for apoptosis) and expression of EF1α (as a loading control) were analyzed by Western blotting analyses in RKO (A) and HCT 116 (B) cells. C and D. Percentage of dead cells detected by Trypan blue dye exclusion staining for RKO (C) and HCT 116 (D) cells. Results are mean ± S.D. (n=3).