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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Cell Physiol. 2012 May;227(5):1805–1813. doi: 10.1002/jcp.22904

Fig. 3. Over expression of sCLU and MDA-7/IL-24 enhances G2/M arrest followed by apoptosis.

Fig. 3

Vec Con-DU-145 and sCLU-DU-145 cells were infected with Ad.mda-7 (50 or 100 pfu/cell) or Ad.vec (100 pfu/cell) and after 48 h cell viability by MTT assay (A) and Caspase-Glo(R) 3/7 assay for caspase-3 expression (B) were determined. C, Colony formation assays in monolayer culture. Colonies were fixed, stained and counted (>50 cells) 2 wks after plating. Values are the mean ± S.D. of three independent experiments. *, P < 0.05 versus Vec Con-DU-145 Ad.mda-7-infected cells. D, After 48 h of infection with Ad.mda-7 or Ad.vec the expression of cyclin B1, cdk1 and PARP was analyzed by Western blotting in Vec Con-DU-145 and sCLU-DU-145 cells.