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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Cancer Res. 2011 Sep 21;71(23):7312–7322. doi: 10.1158/0008-5472.CAN-11-1109

Figure 5. Knockdown of Src impairs transformation of 3T3 cells by ZMYM2-FGFR1.

Figure 5

(A) Src-Yes-Fyn null mouse embryo fibroblasts (SYF−/−) and NIH3T3 (3T3) cells were infected with ZMYM2-FGFR1 or MIEG3 retroviral constructs. Stably infected pools were sorted for GFP positive cells and then analyzed by western blotting with anti-phospho-Src antibodies. The phospho-Src levels are increased in ZMYM2-FGFR1 transformed 3T3 cells. In contrast, total and phospho Src proteins are not detectable in triple knockout SYF−/− cells transduced with ZMYM2-FGFR1. B) Cell proliferation assays demonstrate that ZMYM2-FGFR1 expression increases cell proliferation in NIH3T3, but not in SYF−/− cells. C) Transduction with ZMYM2-FGFR1 significantly promotes anchorage-independent growth of 3T3 cells (3T3-FUS) but not SYF−/− cells (SYF−/−-FUS).