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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Eur J Nucl Med Mol Imaging. 2011 Sep 10;39(1):138–148. doi: 10.1007/s00259-011-1930-x

Fig. 6.

Fig. 6

Immunofluorescence CD105/CD31 double-staining of the 4T1 tumor, liver, spleen, and kidney tissue sections. TRC105 and AlexaFluor488-labeled goat anti-human IgG were used for CD105 staining (green). Afterwards, the tissue slices were stained with rat anti-mouse CD31 antibody and Cy3-labeled donkey anti-rat IgG (red). Note that CD105 expression was high on newly formed blood vessels but not on mature vessels, which exhibited predominantly CD31 staining (indicated by arrowheads). CD105 expression levels in the liver and spleen are much lower than in the tumor vessels. Significant level of CD105 expression was also observed in the renal medulla. All images were acquired under the same condition and displayed at the same scale. Magnification: 200×.