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. 2011 Dec;179(6):2709–2719. doi: 10.1016/j.ajpath.2011.08.029

Figure 3.

Figure 3

Validation of gene array analysis by quantitative real-time PCR. A: List of genes selected to verify the array analysis, including genes representing high- and low-intensity signals and genes that were up- or down-regulated at either the 6- or 24-hour time point. Adj, adjusted; FC, fold change. B: Treatment of myometrial explants with PROK1 for 24 hours up-regulates expression of IL-1β, CCL3, CSF3, and VCAM1, whereas levels of transforming acidic coiled-coil–containing protein 2 remained unaffected. C: Treatment of myometrial explants with 40 nmol/L PROK1 for 6 hours elevated expression of CCL3 and CCL8, whereas there was no difference in the expression of CCL2. *P < 0.05, **P < 0.005, and ***P < 0.0001. n/s, not significant (n = 6). Results are presented as mean ± SEM.