Figure 8.

Targeting the PROK1 gene with miRNA reduces the secretion of inflammatory proteins in response to LPS. Term myometrium was infected with lentivirus containing miRNA constructs targeting PROK1 or a scrambled sequence. Infected explants were subsequently treated with either vehicle or 100 ng/mL of LPS for 24 hours. Results are presented relative to expression in vehicle-treated samples. IL-1β (A), CCL3 (B), and CCL20 (C) protein levels in culture supernatants after treatment with LPS were reduced in tissues infected with PROK1 miRNA compared with tissues infected with a scrambled sequence. *P < 0.05 (n = 5 to 7). Results are presented as mean ± SEM.