Fig. 3.

DNA methylation patterns on chromosomes Bd4 and Bd5 of B. distachyon. a FISH with BAC clones ABR1-26-H1, ABR1-42-H8, ABR1-63-E9 (red fluorescence). b Distribution of 5-MeC signals on the same chromosomes. c–d 5-MeC foci distribution along the longitudinal axes of highly condensed chromosome pair Bd5 excised from the metaphase spread shown on a–b. e–f 5-MeC signal distribution of Bd5 homologues with visible satellite region. g–h 5-MeC foci arrangement of Bd4 homologues. Profiles, idiograms and chromosomes Bd4–Bd5 are oriented with their long arm to the left. Dark green tints on idiograms reflect low methylation level. Methylation profile descriptions as for Fig. 2. DAPI counterstaining, blue fluorescence. Bars 5 μm