Fig. 5.

Characterisation of diastolic Ca²⁺ release events in the absence of ISO a perfusion protocol (upper) and typical records of Fuo-3 fluorescence (lower). b typical line scan images (upper) and corresponding fluorescence profiles (lower) in the presence of (i) 4.75 mmol/L [Ca²⁺]_o and (ii) 4.75 mmol/L [Ca²⁺]_o + 1.0 μmol/L K201; dashed lines (red) represent stimulus mark, circles represent wave initiation points. c mean ± SEM values of (i) number of wave initiation points/diastolic interval and (ii) fluorescence signals measured at the peak and at 950 ms post stimulation. White bars, 4.75: 4.75 mmol/L [Ca²⁺]_o; Grey bars, 4.75 + K201: 4.75 mmol/L [Ca²⁺]_o + 1.0 μmol/L K201; n = 4, *P < 0.05