Fig. 3.

Allele-specific RT-qPCR for quantification of mutated versus wild-type MYH7-mRNA in myocardium. For RT-qPCR, mRNA from cardiac tissue samples was reverse transcribed using a MYH7-specific RT-primer. Subsequent comparative quantitative PCR was accomplished using identical primers and differently labeled mutation or wild-type specific probes in one reaction tube (Online Resource). RT-qPCR data are shown in comparison to data from restriction digest approach performed on the same cardiac samples from left ventricular (LV) anterior wall and right ventricular (RV) wall of H27