Fig. 5.

Fractions of mutated MYH7-mRNA and β-myosin for different FHC mutations. The fractions of mutated mRNA (gray bars) and protein (black bars) in M. soleus are summarized for mutations V606M, I736T, R719W and R723G. Additionally, the R723G-mutated mRNA fractions in myocardium and previously published protein levels of mutations G584R and V606M [26] are depicted. mRNA data shown here are from the restriction digest approach. All data are also listed in Table 1. All mutations significantly deviate from equal abundance of wild-type and mutated transcript with similar deviations also at the protein level. Similar deviations of mRNA and protein levels were found in M. soleus and myocardium for mutation R723G (LV, left ventricle; RV, right ventricle). Note the intra- and/or inter-familial similarity in mutated MYH7-mRNA/protein expression for I736T, R723G and V606M