Fig. 6.

Secondary structure of MYH7 pre-mRNA and mature mRNA. The RNAfold program (minimum free energy) was used to compare the secondary structure of the MYH7 mRNA of mutated and wild-type isoforms. Mutations R719W and I736T generate identical structures as the wild-type sequence and are therefore not depicted. a Secondary mRNA structures of wild-type (NM_000257), mutation V606M (c.1922G>A) and mutation R723G (c.2273C>G). b Secondary structure of the pre-mRNA of wild-type exon 15 to exon 21 (position 6,992–10,380, NG_007884). Enlarged boxes: sites of structural alterations caused by mutations V606M (c.1922G>A, green arrow) and R723G (c.2273C>G, red arrow). Blue arrow in the wild-type sequence: location of mutation R719W that does not alter the pre-mRNA structure