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Withanolide D inhibited angiogenic sprouting in 3D-ECSA. Endothelial cell spheroids from HUVECs and HCECs were treated with 10 ng/mL VEGF in the presence and absence of different concentrations of withanolide D (WD). The HCEC-derived sprouts and HUVEC-derived sprouts were photographed after 36 and 24 hours, respectively.
