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The 20S proteasome inhibitor epoxomicin targets the UPP in HUVECs and HCECs. HUVECs and HCECs were pretreated for 30 minutes with epoxomicin and then stimulated with TNF-α for 20 minutes. Cytoplasmic extracts were analyzed by Western blot for ubiquitin (A, B) and blots reprobed for IκBα expression (C, D). The blots were subsequently reprobed for β-actin to control for protein loading.
