Table 2.
The spectral parameters of the pHLIP-C4phall, pHLIP-C6phall and pHLIP-C10phall in the states I, II and III are presented. The parameters were obtained in the result of the analysis of the fluorescence and CD spectra shown on the Figure 3: the maximum position of the fluorescence spectrum λmax, S – the normalized area under the spectra (normalization was done on the area under the spectrum in the state I); θ222 × 103, deg cm2 dmol-1 res-1 – the molar ellipticity at 222 nm.
| State I | State II | State III | ||
|---|---|---|---|---|
| pHLIP-C4phall | λmax | 350.9 nm | 346.6 nm | 339.7 nm |
| S | 1.0 | 1.08 | 1.05 | |
| θ222 | -4.6 | -4.1 | -6.05 | |
|
| ||||
| pHLIP-C6phall | λmax | 349.9 nm | 348.3 nm | 340.2 nm |
| S | 1.0 | 1.03 | 1.31 | |
| θ222 | -4.6 | -5.2 | -13.4 | |
|
| ||||
| pHLIP-C10phall | λmax | 349.6 nm | 344.6 nm | 339.2 nm |
| S | 1.0 | 1.28 | 1.82 | |
| θ222 | -5.9 | -7.0 | -14.6 | |