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. 2011 Oct 1;34(4):698–706. doi: 10.1590/S1415-47572011005000050

Table 1.

Real-time PCR primer sequences and their respective target genes.

Target gene Primer Sequence Source
Ribosomal protein 49 rp49F 5′-CGTCATATGTTGCCAACTGGT-3′ Lourenço et al., 2008
rp49R 5′-TTGAGCACGTTCAACAATGG-3′
Actin ActMelF 5′-CGGGTGGTGCGATAATCTTG-3′ Judice et al., 2004
actMelR 5′-GGGTATGGAAGCCTGCGGTATC-3′
Transmembrane transporter Mq1F 5′-TTGCTATCTCGTGCGTCTTG-3′ This study
Mq1R 5′-GCGAACATGCCGAATAAACG-3′
Permease Mq2F 5′-CCCGTTAGATGCGACTCAG-3′ This study
Mq2R 5′-CATGCTTGTCCGTTTCATATTG-3′
Ceramide kinase Mq3F 5′-CACGTTCGTGGTGAAGAAGA-3′ This study
Mq3R 5′-CCTCGTGTATCCAATCGTCC-3′
Mq5 Mq5F 5′-GGATTTCAAAGTGGCTGGC-3′ This study
Mq5R 5′-GTTAATCACGTTCAATCGCCC-3′
Dunce DunF 5′-AGCCGACCTGCGACTTCTC-3′ Judice et al., 2006
DunR 5′-ACATGGACATTAGCCCAATGTG-3′
Amino acid-polyamine transporter AmitranspF 5′-AGGGAAGATCCCGTCAAGAA-3′ Judice et al., 2006
AmitranspR 5′-GGGTCGTGTAAAATGCCATGT-3′
Paramyosin PmyosF 5′-ATCCGAGGGAAGATCCAGGTA-3′ Judice et al., 2006
PmyosR 5′-TGCCTCTTGTAGATGCTCATTTTC-3′
Diacylglycerol kinase DGKF 5′-CTTCGTATCGATGCCAGCAA-3′ Judice et al., 2006
DGKR 5′-TTTTGTTGTTCGTCAATCCGTTT-3′
Fatty acid synthase FASF 5′-GATCGCGGGATTGATACCTACT-3′ Judice et al., 2006
FASR 5′-TCGACGGTAACAAAAGTCAAGGA-3′
Groucho GrouF 5′-CGGCGGACGGTTCGA-3′ Judice et al., 2006
GrouR 5′-GATCCCACGAACGCACTGT-3′