Figure 1.

SPT Expression Is Directly Induced by IND.

(A) Expression of a SPT:GUS reporter construct (−1262 bp) at the valve margin of a stage-16 Arabidopsis fruit (Smyth et al. 1990).

(B) and (C) SPT:GUS (−6253 bp) expression in inflorescences of Col-0 (B) and 35S:IND (C).

(D) Q-RT-PCR analysis of SPT relative transcript accumulation after control treatment (−D) or IND activation by Dex treatment (+D) in a 35S:IND:GR line, in presence or absence of Chxe (+CHX). Values are the average of three biological repeats ± sd. +Dex values are significantly different from their corresponding −Dex-treated control (Student’s t test P value < 0.05).

(E) Fold-enrichment of the SPT promoter region in immunoprecipitated chromatin using an anti-GR antibody on the 35S:IND:GR line after control treatment (−D) or Dex treatment (+D). Values are the average of four biological repeats ± sd. The values are significantly different (Student’s t test P value = 0.01).

Bar in (A) = 0.5 mm.