Figure 8.

IND and SPT Cooperate to Regulate Downstream Targets and Bind Separate but Adjacent Elements in the Promoter of PINOID.

(A) Scanning electron microscopy of style and stigmatic tissues in pid-9.

(B) Quantification of yeast one-hybrid interactions between ALC, IND, and SPT proteins and two different elements of PID promoter (E-box variant and Double G-box). ALC was used as a negative control (nonspecific binding of a bHLH protein). Values are averages of five replicates ± sd. Asterisk (*) indicates values that are significantly different from the corresponding ALC control (Student’s t test P value < 0.0001).

(C) and (D) Q-PCR analysis of PID (C) and WAG2 (D) relative to transcript accumulation after control treatment (−D) or IND activation (+D) in 35S:IND:GR (SPT) or 35S:IND:GR spt-12 (spt-12). Values are the average of at least four biological repeats ± se. Asterisk (*) indicates values that are significantly different from their corresponding −Dex-treated control (Student’s t test P value < 0.02).

(E) Schematic of SPT and IND binding to the PID promoter. The double G-box elements are orange and the E-box is green. TATA indicates the position of the transcription initiation TATA box, and the PID coding region is shown in red.

Bar in (A) = 100 μm.