E2Fa Is Involved in Lateral Root Initiation.
(A) to (C) E2Fa, E2Fb, and E2Fc expression kinetics upon synchronous lateral root initiation in roots of Col-0 (A), slr-1 (B), and in pericycle-sorted cells (C). Data represent mean ± se (n ≥ 2).
(D) to (G) E2Fa-GFP protein accumulation upon NAA (5 μM) application after 1 h (D) or 5 h (E) in XPP cells counterstained with PI. E3Fa-GFP is visible before (6 h) (F) and after the first asymmetric division (7 h) (G) marking lateral root initiation. Bar = 50 μm (all photomicrographs are at the same magnification).
(H) Quantification of emerged (E), nonemerged (NE), and total (T) lateral root primordium density of e2fa T-DNA insertion lines 8 d after germination.
(I) Detailed staging of lateral root stage density of e2fa T-DNA insertion lines 8 d after germination. Data represent mean ± se (n ≥ 12; *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001; two-sided t test).