Figure 2.

NOI Domains Are Required for Defense-Suppressing Activity of RIN4.

(A) Schematic diagram of RIN4 derivatives as in Figure 1. ΔΔNOI lacks amino acids 1 to 64 and 149 to 176. w.t., wild type.

(B) and (C) Callose induced by flg22 infiltration (B) and growth of hrcC (C) in Col-0 and lines of Col-0 inducibly expressing the indicated RIN4 derivatives as in Figure 1. *P < 0.005; **P < 0.05 [two-tailed Student’s t test for comparison with Col-0 plants].

(D) Immunoblots with anti-T7 antisera to compare levels of RIN4 derivatives as in Figure 1.