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. 2011 Oct 7;23(10):3798–3811. doi: 10.1105/tpc.111.088708

Figure 5.

Figure 5.

Derivatives of RIN4 Suppress PTI More Potently When Not Membrane-Tethered.

(A) Expression of RIN4 derivatives in rpm1 rps2 rin4 plants was induced by spraying with Dex 48 h before infiltration with 30 μM flg22. Callose numbers were counted from plants expressing comparable levels of RIN4FL, CCC>AAA, and 177Δ211 proteins (see Supplemental Figure 8 online). The results shown are averaged from three independent biological replicates, with the average number of callose deposits induced in plants expressing RIN4FL in each experiment normalized to 1. Error bars represent sd. *, P = 0.001; **P < 0.0001 [two-tailed Student’s t test for comparison with rpm1 rps2 rin4 plants expressing RIN4FL].

(B) Expression of RIN4 derivatives in Col-0 or rpm1 rps2 rin4 plants was induced by spraying with Dex 48 h before infiltration with 105 CFU/mL of hrcC. Growth data in rpm1 rps2 rin4 was obtained from plants expressing similar, low levels of RIN4FL and derivatives (see Supplemental Figure 9 online). Numbers of hrcC were determined 0 (gray bars), 2 (white bars), and 4 (black bars) d after infiltration. Error bars represent sd. *P < 0.05 [two-tailed t test for comparison with rpm1 rps2 rin4 plants]; **P < 0.005 [two-tailed Student’s t test for pairwise comparison with Col-0 plants].