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. 2011 Oct 26;31(43):15362–15375. doi: 10.1523/JNEUROSCI.3181-11.2011

Figure 6.

Figure 6.

Cell-autonomous expression of irx-1 is required for synaptic remodeling in unc-55 VD motor neurons. A–D, SNB-1::GFP puncta label GABAergic NMJs. mCherry marks cells expressing the control wdEx658 transgenic array (A, C) or specific neurons expressing the irx-1(csRNAi) transgene, wdEx686 (B, D) (see Materials and Methods). A, B, Dorsal nerve cord. C, D, Ventral nerve cord. Common region of single adult animal in A and C, and separate adult in B and D. Scale bars, 10 μm. A, Note dense array of SNB-1::GFP puncta in the dorsal nerve cord of unc-55 adult. B, Fewer SNB-1::GFP puncta are observed in the region corresponding to dorsal arm of irx-1(csRNAi) expressing VD motor neuron (marked with mCherry). Note that dorsal SNB-1::GFP puncta from DD3 in this region do not overlap with the VD6 mCherry marker. C, SNB-1::GFP puncta are depleted from the ventral nerve cord of unc-55 adults. D, Expression of irx-1(csRNAi) in VD7 (marked with mCherry) of an unc-55 mutant restores ventral SNB-1::GFP puncta. SNB-1::GFP puncta are absent from neighboring VD6 motor neuron that does not express irx-1(csRNAi). E, The dotted lines denote anterior regions of mCherry-marked VD motor neurons in which SNB-1::GFP puncta were counted (see Materials and Methods). F, Quantification of irx-1(csRNAi) suppression of the Unc-55 synaptic remodeling defect. **p < 0.0001, two-way ANOVA with Bonferroni's multiple-comparison test. N = 50 VD anterior regions per genotype.