FIG. 2.

Effects of gomisins on P. gingivalis (P.g) lipopolysaccharide (LPS)-induced production of pro-inflammatory cytokines in Raw264.7 cells. Cells were incubated with various concentrations of gomisins for 1 hour prior to a 16-hour exposure to LPS (1 μg/mL). We measured (A) tumor necrosis factor-α (TNF-α), (B) interleukin (IL)-1β, and (C) IL-6 in the culture supernatant by enzyme-linked immunosorbent assay. Data are mean±SE values from three independent experiments in each group. *P<.05 versus the LPS-treated group.