Figure 10.
Uptake of radiolabeled inositol and serine from the medium. Y700pGAL1-ACB1 and Y700 were grown to early log phase in the presence of inositol, harvested, and resuspended in PBS to an OD of 1.0. (For details, see MATERIALS AND METHODS). After addition of radiolabeled inositol (A) or serine (B), uptake was stopped by transferring the cells to ice-cold PBS containing excess unlabeled inositol or serine, at the times indicated. Cells were collected by vacuum filtration, the filter paper was dried (for details, see MATERIALS AND METHODS), and the 3H activity of each cell spot was quantified by phosphorimaging.