Figure 11.
Test of cell envelope integrity. (A) Y700pGAL1-ACB1 and Y700 were diluted to OD 0.1 and grown in the presence of different concentrations of the detergent Brij58. After 16 h the OD600 of each culture was determined. (B) Y700pGAL1-ACB1 and Y700 were resuspended to OD 0.5 and treated with Zymolyase while the optical density was monitored. Data shown ± SEM (n = 3).