Figure 2.

(A) Acyl-CoA composition of Y700 (black columns) and Y700pGAL1ACB1 (open columns). The cells were grown in YNB-glucose for 24 h, diluted into fresh YNB-glucose medium to OD 0.05, grown to OD 0.2, harvested, and analyzed as described in MATERIALS AND METHODS. Values are means ± SEM of four independent experiments. (B) Total fatty acid composition of Y700 (black columns) and Y700pGAL1-ACB1 (open columns). The cells were analyzed as described for A. Values are means ± SEM of six independent experiments and indicate total amounts of fatty acids/absorbance units of cells. UFA, unsaturated fatty acid (C14:1+C16:1+C18:1).