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Determination of yeast ACBP ligand-binding specificity by isoelectric focusing. Yeast ACBP was incubated with threefold excess of different acyl-CoAs and focused on an isoelectric focusing gel. Binding of acyl-CoA is identified by band shift. Lane 1, without ligand; lane 2, C16:0-CoA; lane 3, C18:0-CoA; lane 4, C20:0-CoA; lane 5, C22:0-CoA; lane 6, C24:0-CoA.
