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Incorporation of [3H]myo-inositol into PI and sphingolipids. (A) Cells were pulse labeled for 15 min and chased for 5 h. Cells were harvested at the indicated times, lipids were extracted, and total [3H]myo-inositol incorporation was determined by scintillation counting. (B) After mild alkaline hydrolysis and re-extraction, the amount of [3H]myo-inositol in the sphingolipids was determined. Data are means (±SEM) of three independent determinations.
