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. Author manuscript; available in PMC: 2011 Dec 2.
Published in final edited form as: Cancer Res. 2009 Oct 6;69(20):7911–7916. doi: 10.1158/0008-5472.CAN-09-1287

Figure 2.

Figure 2

AIP4 interacts with FLIPS in a PTEN- and ubiquitin-dependent manner. PTEN WT or mutant cells were transfected with a blank vector or a construct encoding HA-ubiquitin (C) or exposed to vehicle (B) or TRAIL (800 ng/mL, 24 h; A). Cells were then lysed and immunoprecipitated using antibodies specific for IgG or for DR5 (A), AIP4 or FLIPS (B), or AIP4 (C). Lysates and immunoprecipitates were then subjected to Western blot analysis of AIP4 and DR5 (A), FLIPS and AIP4 (B), and AIP4 and HA-AIP4 (C) levels.