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Urea denaturation of purified profilins monitored by intrinsic fluorescence. (A) Mutants that are equal to or more stable than C89S (●) were considered as stable, including poly-l-proline binding mutants Y5A (▵), Y5D (□), Y120D (▪), Y120A (▾), Y126D (○) and actin binding mutants Y79R (▿), K81E (⋄), K81Y (−), P107Y (+), A111E (♦), and wild-type profilin (▴). (B) Unstable mutants E42K(□) and L121R (○) denature at lower urea concentrations than C89S (●) and wild-type profilin (▴).
