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Dying M. tuberculosis-infected DCs secrete cytokines. DCs were infected with live/dead Mtb H37Ra at MOI 1 for 24 h or 48 h, or treated with LPS (1 μg/ml) for 24 h. (U = uninfected, LH37Ra = live H37Ra, sH37Ra = streptomycin-killed H37Ra.) Cytokine levels were measured in cell-free supernatants by ELISA. Data were analysed using the Friedman test followed by Dunn's Multiple Comparison test and represent the means (± SEM) of 3 individual donors.
