Skip to main content
. 2011 Dec 2;6(12):e27493. doi: 10.1371/journal.pone.0027493

Table 1. Primers used for PCR and sequencing.

Gene Primer sequences Annealing temp. for PCR
per F 5′-TTCTGCTGCGTCATCTCCATGC-3′ 51°C
R 5′-GGAACTCTTACTGTCATAGTAGTC-3′
S 5′-GACTTTGTGCACATCAAGGA-3′
tim F 5′-TGATACCVYTGCTGGAGAATGCC-3′ 63°C
R 5′-TNGTGTCDATGTGCTCCATRTCC-3′
S 5′-GAACCAGGAGTCCATCTCCA-3′
Clk F 5′-TGGTCAARTTYGTKGGYTACTTTC-3′ 55°C
R 5′-SGCATAGCTGACMACYTTRTGRGTG-3′
S 5′-GAAATGAGCATYATYGATCCSAC-3′
cyc F 5′-CAAAAYCACAGYGAGATCGAGAARCG-3′ 53°C
R 5′-ATRTAGTCAATYTCGYTYGTCC-3′
S 5′-TGAGCTCTCCTCCATGATCC-3′
cry F 5′-AGCGAATGTGATCTGGTTCC-3′ 50°C
R 5′-ATGCTCAGGCAGATCTCGTT-3′
S 5′-ACACAGGCTCGCAACTGGAC-3′

F, forward primer; R, reverse primer; S, sequence primer.