Table 1. Primers used for PCR and sequencing.
Gene | Primer sequences | Annealing temp. for PCR | |
per | F | 5′-TTCTGCTGCGTCATCTCCATGC-3′ | 51°C |
R | 5′-GGAACTCTTACTGTCATAGTAGTC-3′ | ||
S | 5′-GACTTTGTGCACATCAAGGA-3′ | ||
tim | F | 5′-TGATACCVYTGCTGGAGAATGCC-3′ | 63°C |
R | 5′-TNGTGTCDATGTGCTCCATRTCC-3′ | ||
S | 5′-GAACCAGGAGTCCATCTCCA-3′ | ||
Clk | F | 5′-TGGTCAARTTYGTKGGYTACTTTC-3′ | 55°C |
R | 5′-SGCATAGCTGACMACYTTRTGRGTG-3′ | ||
S | 5′-GAAATGAGCATYATYGATCCSAC-3′ | ||
cyc | F | 5′-CAAAAYCACAGYGAGATCGAGAARCG-3′ | 53°C |
R | 5′-ATRTAGTCAATYTCGYTYGTCC-3′ | ||
S | 5′-TGAGCTCTCCTCCATGATCC-3′ | ||
cry | F | 5′-AGCGAATGTGATCTGGTTCC-3′ | 50°C |
R | 5′-ATGCTCAGGCAGATCTCGTT-3′ | ||
S | 5′-ACACAGGCTCGCAACTGGAC-3′ |
F, forward primer; R, reverse primer; S, sequence primer.