Figure 1. Tissue-specific expression patterns of mRNAs of the main components of βAR signaling in the dLGN of Long Evans rats.

RT-PCRs of mRNA from whole brain extracts (A) and VB (B) show that the α1C subunit (colored squares) as well as other Ca²⁺ channels forms are widely expressed. The skeletal muscle form α1S was not found in both regions (as well as in dLGN, see Figure F). In all experiments, appropriate markers for size were used in order to assure detection of specific forms of Ca²⁺ channels. (C) Using specific primers for all three types of βAR, we have shown specific expression of only β₁ and β₂AR. β₃AR was neither expressed in VB nor in dLGN. (D) Following the same procedure, we have shown the expression of some of the G proteins coupled with metabotropic receptors (Gα and Gi) in dLGN. In all experiments specific markers for size were used. (E) Expression of three different adenylate cyclases, namely type 1, 6 and 8 with two isoforms A/B and C in dLGN.