Figure 3.

The effect of DEC cytoplasmic domain derivatives on the organization of adherens junctions and subcellular distribution of β-catenin (β-cat). MDCK cells were transfected with various GFP-tagged DEC derivatives (diagrammed in Figure 1, A and B), and the distribution of the GFP-tagged DEC derivatives (A, C, E, G, and I) and of the endogenous β-catenin (B, D, F, H, and J) was determined by double fluorescence microscopy using rhodamine-labeled anti-β-catenin antibody. Bar (in A), 10 μm. Note the reduction in junctional β-catenin in DEC-expressing cells but not in cells transfected with other DEC constructs. Also note that DEC9 and DEC29 do not increase the endogenous β-catenin level, whereas DEC13 does.