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. 2011 Dec 2;6(12):e27955. doi: 10.1371/journal.pone.0027955

Figure 6. GATA1 and CBP bind to GATA1-HS2-containing 5′ LTRs.

Figure 6

HEL cells were transduced with GLOBE and G-GLOBE and ChIP assay was performed using antibodies against GATA1 and CBP. (A) Schematic map of GLOBE and G-GLOBE vectors. Arrows indicate the primer pairs used to amplify by PCR the viral 5′ LTR. (B) Chromatin was immunoprecipitated with antibodies against GATA1 and CBP and isotype-matched control antibodies (Rabbit IgG). GATA1 and CBP binding to the 5′LTR of GLOBE and G-GLOBE vectors was measured by real-time PCR. GATA1 and CBP are detected only at the GATA1-HS2-containing 5′LTRs (n = 2). *P<0.05 by Student t test. Error bars represent SEM.