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. 2011 Dec 2;6(12):e27955. doi: 10.1371/journal.pone.0027955

Figure 7. Increased CBP-mediated histone acetylation levels at GATA1-HS2-containing LV.

Figure 7

ChIP assay was carried out in GLOBE- and G-GLOBE-transduced HEL cells using antibodies against pan-acetylated histone H3, acetylated histone H3K18, acetylated histone H4K5, and acetylated histone H4K8. (A) Map of GLOBE and G-GLOBE proviruses Arrows indicate the primer pairs used to amplify the 5′LTR and the β-globin promoter-HS2 region. The acetylation levels are significantly higher at the 5′ LTRs (B) and at the β-globin promoter region (C) of GATA1-HS2-containing LV compared to the GLOBE vector. (n = 4). *P<0.05; **P<0.01 by Student t test. Error bars represent SEM.