Table 1. The 24 most represented transcripts in the A. ostenfeldii EST library.
no. of TCs | no. of ESTs | % of total ESTs | Annotation |
31 | 355 | 2.89 | 28S rRNA |
37 | 265 | 2.16 | cytochrome b |
21 | 249 | 2.03 | cytochrome c oxidase subunit 1 |
5 | 185 | 1.51 | peridinin-chlorophyll a-binding protein |
2 | 185 | 1.51 | potassium channel |
15 | 81 | 0.66 | cytochrome c oxidase subunit 3 |
7 | 43 | 0.35 | cytochrome b and cytochrome c oxidase subunit 3 |
7 | 29 | 0.24 | luciferin-binding protein |
2 | 25 | 0.20 | 18S rRNA |
8 | 22 | 0.18 | calcium-dependent protein kinase |
2 | 21 | 0.17 | ubiquitin |
3 | 19 | 0.15 | heat shock protein 70 |
4 | 16 | 0.13 | alcohol dehydrogenase, zinc-containing |
1 | 15 | 0.12 | elongation factor EF1A |
1 | 15 | 0.12 | hypothetical protein |
2 | 15 | 0.12 | voltage-dependent T-type calcium channel |
5 | 14 | 0.11 | actin |
3 | 14 | 0.11 | heat shock protein 90 |
6 | 14 | 0.11 | serine/threonine-protein kinase |
1 | 13 | 0.11 | elongation factor EF-G |
3 | 13 | 0.11 | ribonucleoside-diphosphate reductase small chain |
1 | 12 | 0.10 | hypothetical protein |
1 | 12 | 0.10 | hypothetical protein |
4 | 12 | 0.10 | Pentatricopeptide (PPR) repeat-containing protein |
All TCs (tentative consensus sequences = clustered and assembled ESTs using TIGR clustering-algorithm, and cap3) having the same annotation were added and the number of ESTs as well as the percentage of the total number of ESTs is shown. Annotations were allocated when at least one BLAST hit to one of the databases had an e-value<10−30.