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. 2011 Dec 2;6(12):e27979. doi: 10.1371/journal.pone.0027979

Figure 2. The Crr1 response regulator is required for the resistance of cells to hydrogen peroxide.

Figure 2

2×103 cells, and 10-fold dilutions thereof, of exponentially-growing wild-type (WT, JC806), crr1Δ (JC566), crr1Δ+CRR1 (JC803, JC804), ssk1Δ (JC784), ssk1Δ/crr1Δ (JC787), hog1Δ (JC50) and hog1Δ+HOG1 (JC52) strains were spotted onto YPD plates containing the following agents; hydrogen peroxide (2, 2.5, 3, 3.5 mM), 250 mM menadione, 2.5 mM Na arsenite, NaCl (0.5, 1.0 M), sorbitol (1.0, 1.2 M), 0.6 M KCl, 12.5 mM caffeine, 1 mM cadmium, 5 µg/ml fluconazole, 5 µg/ml nystatin, 0.02% SDS, 50 µg/ml Calcofluor White and 200 µg/ml Congo Red. Plates were incubated at 30°C for 24 h.