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. 2011 Dec 2;6(12):e27979. doi: 10.1371/journal.pone.0027979

Figure 3. The Ssk1, but not the Crr1, response regulator is required for Hog1 activation in response to hydrogen peroxide.

Figure 3

Western blot analysis of whole cell extracts isolated from wild-type (WT, JC806), crr1Δ (JC566), ssk1Δ (JC784), and crr1Δssk1Δ (JC787) cells after treatment with 5 mM hydrogen peroxide or 1 M NaCl for the specified times. Western blots were probed with an anti-phospho-p38 antibody, which specifically recognises the phosphorylated, active form of C. albicans Hog1 (Hog1-P). Total levels of Hog1 protein were determined by stripping the blot and reprobing with an anti-Hog1 antibody which recognises both phosphorylated and unphosphorylated forms of Hog1 (Hog1).