Table 2.

Primers for RT-PCR and sequence analyses of CDV N, P and H genes

Gene Primer	Sequence (5'-3')	Nucleotide position
N Upper	AACAA GGCTA GGGTT CAGAC CT	80-102
N Lower	TTGTT GACTG ATGCA AGACT GGT	1688-1711
P Upper	CGACC ACCCG TTCTA TC	1779-1796
P Lower	GCGGA CTTAG GCTCT TGT	3404-3422
H Upper	CTTAG GGCTC AGGTA GTCCA	7056-7076
H Lower	ATTCA ATCGT CTGTA AGGGA	8957-8977

*Design the primers based on Onderstepoort strain of canine distemper virus