Figure 8. Physicochemical study of structural modification of spectrin_VL.

A. CD-spectra. Far-UV CD spectra of spectrin_VL and spectrin_N in phosphate buffer (20 mM, pH 7.0) indicating the molar residue ellipticity as a function of wavelength along with the buffer only. B. Binding of ¹²⁵I-spectrin to spectrin-depleted IOV_N. Various concentrations of ¹²⁵I-spectrin_VL/N were incubated with a constant amount of spectrin-depleted-IOV_N followed by determination of specific binding as described in Materials and Methods.