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. 2011 Dec 2;6(12):e28351. doi: 10.1371/journal.pone.0028351

Figure 7. Individual GG(D/E)EF/EAL domain proteins affect S. typhimurium induced IL-8 production by the gastrointestinal cell line HT-29.

Figure 7

Relative IL-8 production of HT-29 cells after incubation with individual GG(D/E)EF/EAL mutants. IL-8 production of the wild type (WT) S. typhimurium UMR1 was set 100%. ΔfliCΔfljB, negative control, U = unstimulated cells. Strains were grown under invasion inducing conditions (standing culture, LB+0.3M NaCl) until O.D.600 0.6 and co-incubated with HT-29 cells for 1 h. IL-8 production of HT-29 cells was measured by ELISA. Bars show mean % ± standard deviation from at least three independent biological experiments performed in two technical replicates. Statistical significance is indicated by *P<0.05, **P<0.01, ***P<0.001 as compared with wild type S. typhimurium UMR1.