Table 1. Primers sequence, annealing temperatures and size of PCR products used to amplify and sequence exons of the G6PD gene.

Exons	Primer name	Sequence (5′-3′)	Hybridization T°	Size of PCR products
Exon 2	PCR_G6PD_Ex2_F	TGAAGGCTGCCTAGGAGAGA	58°C	494 bp
	PCR_G6PD_Ex2_R	CAGGTAGAGCCGGGATGAT
Exons 3–4	PCR_G6PD_Ex3–4_F	TGTCCCCAGCCACTTCTAA	58°C	400 bp
	PCR_G6PD_Ex3–4_R	GGAGAGGAGGAGAGCATCC
Exon 5	PCR_G6PD_Ex 5_F	CGGGGACACTGACTTCTG	56°C	500 bp
	PCR_G6PD_Ex 5_R	ACGCTGCCACCTTGTGGT
Exons 6–7	PCR_G6PD_Ex 6–7_F	ACACAAGGCACGGGAGGT	58°C	697 bp
	PCR_G6PD_Ex 6–7_R	GAGGAGCTCCCCCAAGATAG
Exon 8	PCR_G6PD_Ex 8_F	CCCTTGAACCAGGTGAACAG	58°C	221 bp
	PCR_G6PD_Ex 8_R	TCAGTGCCTCGTCACAGATG
Exon 9	PCR_G6PD_Ex 9_F	CCTGAGGGCTGCACATCT	58°C	363 bp
	PCR_G6PD_Ex 9_R	GTGCGTGAGTGTCTCAGTGG
Exons 10–11–12	PCR_G6PD_Ex 10–12_F	TGAGACACTCACGCACTGGT	58°C	752 bp
	PCR_G6PD_Ex 10–12_R	TGAGGTAGCTCCACCCTCAC
Exon 13	PCR_G6PD_Ex 13_F	TTATGGCAGGTGAGGAAAGG	58°C	848 bp
	PCR_G6PD_Ex 13_R	GAAGTGGGTCCTCAGGGAAG