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. 2011 Dec 2;6(12):e28352. doi: 10.1371/journal.pone.0028352

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Correia et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Engineered bone grafts were evaluated for the expression of vascular and osteogenic proteins. CD31 (black arrows) and vWF (indicated by *) expression was observed in constructs from all groups. Vascular structures were most developed in EGM|cocktail and EGM|cocktail+MSC groups. Collagen I and BSP were readily apparent in the OM group. Expression was observed in individual cells in EGM group, but not distributed through matrix. Both collagen I and BSP were observed in all other groups. In EGM|cocktail+MSC group, BSP appeared in close proximity to the vascular structures. Scale bar = 20 µm.