Table 1. TS and neutral mutations of five proteins.

Protein	Permissive/restrictive temperatures	PDB	TS	Neutral	Total
T4 lysozyme	25°C/37°C	2LZM_A	95	1688	1783
lac repressor	25°C/37°C	1EFA_A	159	2534	2693
CcdB	30°C/37°C	3VUB_A	219	1011	1230
TBP	30°C/37°C	1YTF_A	141	0	141
p53	30°C/37°C	1TUP_B	133	251	384
Total	-	-	747	5484	6231